MCW BioMart
 

RGD Cookbook

Links

 RGD: Building a list of Microsatellite Markers
            from a Genomic Region
 
 version 0.6

Problem:

You have a region of the rat genome identified (chr12: 9194687 - 39194687) and you want to know what microsatellite markers lie within that region.

Solution:

The first step is to select the RGD Microsatellite Markers database from the martveiw page.
(Click Here to open Martview in a new window.)

rgd_front

We then click on "Filters" from the side bar. This will bring us to our filters page. We check the first box, select chromosome 12 and enter in the start and stop position in base pair value for our region.

uniprot filter

We now click on "Attributes" from the side bar to go to the attributes page. We select RGD ID, Symbol, and Start and Stop. We then click the Results button. This will return a preview of all the markers that are wholey or partially fall in our region.

rgd attributes

Discussion

The SSLP Biomart can also be used to export the size of a microsatellite marker for a given rat strain. This can be used to indentify polymorphic markers between two or more inbred strains so that these markers can be used in genetic mapping studies. If you are interested in the selection of polymorphic markers or a more graphical view of the allele data in different starins please have a look at the RGD Genome Scanner and ACP Haplotyper tools. These use the same microsatellite allele data and provide a custom web interface specifically designed to address these common research problems.